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Home >> Antibodies >> CCR4-NOT transcription complex, subunit 10 (CNOT10)

CCR4-NOT transcription complex, subunit 10 (CNOT10)

Mouse Monoclonal Antibody [Clone PCRP-CNOT10-1D5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis Purified CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using CNOT10 Mouse Monoclonal Antibody (PCRP-CNOT10-1D5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CNOT10-Monospecific Mouse Monoclonal Antibody (PCRP-CNOT10-1D5).

Product Details

Synonyms

FLJ12890, FLJ13165

Positive Control

HeLa or HepG2 cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CNOT10 protein

Cellular Localization

Cytoplasm. Mitochondrion and Nucleus, PML body.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c

Mol. Weight of Antigen

82.31kDa

Specificity & Comments

CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 25904
  •  Gene Symbol: CNOT10
  •  SwissProt: Q9H9A5
  •  Chromosome Location: 3p22.3
  •  Unigene: 444851