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CCR4-NOT transcription complex, subunit 10 (CNOT10) Antibody [PCRP-CNOT10-1D5]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
25904-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
25904-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
25904-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml

Summary

CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2c /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
Positive Control
HeLa or HepG2 cells.
Immunogen
Recombinant full-length human CNOT10 protein
Alternate Names
CCR4-NOT transcription complex subunit 10, FLJ12890, FLJ13165

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-CNOT10-1D5
Chromosome Location
3p22.3
Mol. Weight of Antigen
82.31kDa

Functions

  • Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.

Key References

  • Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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