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Home >> Antibodies >> Carbonic Anhydrase IX (Renal Cell Marker)

Carbonic Anhydrase IX (Renal Cell Marker)

Mouse Monoclonal Antibody [Clone CA9/781]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CAIX Mouse Monoclonal Antibody (CA9/781).

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SDS-PAGE Analysis Purified CAIX Mouse Monoclonal Antibody (CA9/781). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of HCT116 cell lysate CAIX Mouse Monoclonal Antibody (CA9/781).

Product Details

Synonyms

CA-IX, CA9, CAH9_HUMAN, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Positive Control

Normal kidney or renal cell carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CA9 protein

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human. Horse.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sly, W.S., et al. 1995. Annu. Rev. Biochem. 64: 375-401.

Bioinformatics

  •  Entrez Gene ID: 768
  •  Gene Symbol: CA9
  •  SwissProt: Q16790
  •  Chromosome Location: 9p13.3
  •  Unigene: 63287
NBT00781