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Home >> Antibodies >> Blood Group Antigen H Type 2 (CD173)

Blood Group Antigen H Type 2 (CD173)

Mouse Monoclonal Antibody [Clone 19-OLE]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen H Type 2 Monoclonal Antibody (19-OLE)

Product Details

Synonyms

A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT

Positive Control

KG1 cells. Human colorectal carcinoma.

Known Applications & Suggested Dilutions

IF, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Mucinous colonic adenocarcinoma

Cellular Localization

Cell Surface & Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

Multiple

Specificity & Comments

Recognizes the blood group H type 2 antigens, trisaccharide Fuc1 of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blood transfusion and immunohaematology, Ph Rouger, D Anstee and Ch Salmon (Eds), Arnette, France 30 (5), p. 353-720, 1987.
  2. Norwalk Virus Binds to Histo-Blood Group Antigens Present on Gastroduodenal Epithelial Cells of Secretor Individuals. SEVERINE MARIONNEAU, NATHALIE RUVOE N, BEATRICE LE MOULLAC-VAIDYE, MONIQUE CLEMENT, ANNE CAILLEAU-THOMAS, GUILLERMO RUIZ-PALACOIS, PENGWEI HUANG, XI JIANG, and JACQUES LE PENDU. GASTROENTEROLOGY 2002;122:1967-1977.
  3. Bara J, Daher N, Mollicone R, Oriol R. Immunohistological patterns of 20 monoclonal antibodies against non-A, non-B glycoconjugates in normal human pyloric and duodenal mucosae. Blood TransfImmunohae- matol. 1987; 30:685-692.
  4. Expression of Mucin Peptide and Blood Group ABH- and Lewis-Related Carbohydrate Antigens in Normal Human Conjunctiva. Catherine Garcher, Jacques Bara, Alain Bron, and Rafael Oriol. Invest Ophthalmol Vis Sci. 1994;35:1184-1191.

Bioinformatics

  •  Entrez Gene ID: 28
  •  Gene Symbol: ABO
  •  SwissProt: P16442
  •  Chromosome Location: 9q34.2
  •  Unigene: 654423
NBT00947