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Blood Group Antigen H Type 2 (CD173) Antibody [A46-B/B10]

In Stock
Catalog Number Formulation Size Price
28-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
28-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
28-MSM2-P2
Purified Ab with BSA and Azide at 200ug/ml
200ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes the blood group H type 2 antigens, trisaccharide Fuc伪1-2Gal尾1-4GlcNAc尾1 of human origin. The specificity of this MAb has been identified by haemagglutination tests, immunohistochemistry, binding inhibition studies, and absorption experiments performed with synthetic oligosaccharides. The antibody is virtually nonreactive with H type 1 antigen or with closely related type 2 structures (e.g., Y antigen). A46-B/B10 strongly agglutinates human erythrocytes according to the amount of H substance expressed and can, therefore, easily discriminate between blood groups A1 and A2 as well as A1B and A2B (A1 and A1B are not or only weakly agglutinated). In immunohistochemistry, this antibody seems to provide a highly specific reagent for a restricted number of carcinomas and epithelial lineages in tissue sections and in vitro. This was tested on 753 blood samples. It strongly agglutinates human erythrocytes of blood groups 0, A2 and A2B, but not or only very weakly A1 and A1B. This MAb promises to be useful in the determination of A and AB subgroups in blood group serology. This MAb might also be able to define B subgroups. Among 263 B blood samples, 45 (17.1%) were negative or only weakly positive with A46-B/B10.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Golgi apparatus, Golgi stack membrane, Secreted
Gene Name
ABO
Positive Control
KG1 cells or human colorectal carcinoma tissues.
Immunogen
Human breast cancer MCF-7 cells
Alternate Names
Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT

Database Links

Entrez Gene ID
28
SwissProt

Additional Information

Clone
A46-B/B10
Chromosome Location
9q34.2
Mol. Weight of Antigen
Multiple

Functions

  • This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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