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Home >> Antibodies >> Bcl-6 (Follicular Lymphoma Marker)

Bcl-6 (Follicular Lymphoma Marker)

Mouse Monoclonal Antibody [Clone SPM602]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with bcl-6 Monoclonal Antibody (SPM602).

Product Details

Synonyms

B-cell lymphoma 5 protein; B-Cell Lymphoma 6 Protein; BCL5; BCL6; BCL6A; cys his2 zinc finger transcription factor; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S

Positive Control

Raji or Ramos cells. Tonsil or Hodgkin's lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.125-0.25ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human bcl-6 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

95kDa

Specificity & Comments

Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Pasqualucci, L., et al. 2003. Leuk. Lymphoma 44 Suppl 3: S5-12.
  2. Garcia JF, et al. 2006. J. Histochem Cytochem. 54:31.
  3. Ree, H.J., et al. 2003. Hum. Pathol. 34: 610-616.

Bioinformatics

  •  Entrez Gene ID: 604
  •  Gene Symbol: BCL6
  •  SwissProt: P41182
  •  Chromosome Location: 3q27.3
  •  Unigene: 478588
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