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Home >> Antibodies >> BCL2-like 2 (BCL2L2) / BCL-W

BCL2-like 2 (BCL2L2) / BCL-W

Mouse Monoclonal Antibody [Clone PCRP-BCL2L2-1A4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified BCL2L2 Mouse Monoclonal Antibody (PCRP-BCL2L2-1A4). Confirmation of Purity and Integrity of Antibody.

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Flow cytometric analysis of PFA-fixed Raji cells. BCL2L2 Mouse Monoclonal Antibody (PCRP-BCL2L2-1A4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained using BCL2L2 Mouse Monoclonal Antibody (PCRP-BCL2L2-1A4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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Immunofluorescence Analysis of PFA-fixed U87 cells stained using BCL2L2 Mouse Monoclonal Antibody (PCRP-BCL2L2-1A4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using BCL2-like 2 Mouse Monoclonal Antibody (PCRP-BCL2L2-1A4).

Product Details

Synonyms

BCL-W, BCL2-L-2, BCLW, KIAA0271, PPP1R51

Positive Control

PC-3, HEK293, HePG2, MCF-7 or U87 cells.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length BCL2L2 protein

Cellular Localization

Cytoplasm. Mitochondrion membrane.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

22-30kDa

Specificity & Comments

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.
  2. O Reilly, L. A. et al. Tissue expression and subcellular localization of the pro-survival molecule Bcl-w.Cell Death Differ.8, 486 494 (2001).

Bioinformatics

  •  Entrez Gene ID: 599
  •  Gene Symbol: BCL2L2
  •  SwissProt: Q92843
  •  Chromosome Location: 14q11.2-q12
  •  Unigene: 410026