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Home >> Antibodies >> B7-H4 (Immuno-Inhibitory Protein)

B7-H4 (Immuno-Inhibitory Protein)

Recombinant Rabbit Monoclonal Antibody [Clone B7H4/2652R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Rabbit Recombinant Monoclonal Antibody (B7H4/2652R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of SKBR-3 cells using B7-H4 Rabbit Recombinant Monoclonal Antibody (B7H4/2652R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with B7-H4 Rabbit Recombinant Monoclonal Antibody (B7H4/2652R).

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SDS-PAGE Analysis Purified B7-H4 Rabbit Recombinant Monoclonal Antibody (B7H4/2652R). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7S1; Immune costimulatory protein B7-H4; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1

Positive Control

HeLa, SKBr-3, MCF-7 cells. Ovary, Pancreas, Placenta or Spleen.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (1-2ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)

Cellular Localization

Cell Surface & Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

35kDa

Specificity & Comments

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sica, G.L., et al. Immunity 18:849-861.
  2. Salceda, S., et al. Exp. Cell Res. 306:128-141.

Bioinformatics

  •  Entrez Gene ID: 79679
  •  Gene Symbol: VTCN1
  •  SwissProt: Q7Z7D3
  •  Chromosome Location: 1p13.1-12
  •  Unigene: 546434
NBT02652