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Home >> Antibodies >> 8-Oxoguanine DNA Glycosylase

8-Oxoguanine DNA Glycosylase

Mouse Monoclonal Antibody [Clone CPTC-OGG1-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Skin stained with 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1).

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SDS-PAGE Analysis Purified 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

HMMH, HOGG1, MUTM, OGH1

Positive Control

HeLa or Jurkat cells. Kidney, skin or lymph node.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human OGG1 protein

Cellular Localization

Mitochondrion. Nucleus speckle.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

39kDa

Specificity & Comments

8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Cheng, K.C., et al. 1992. J. Biol. Chem. 267: 166-17

Bioinformatics

  •  Entrez Gene ID: 4968
  •  Gene Symbol: OGG1
  •  SwissProt: O15527
  •  Chromosome Location: 3p25.3
  •  Unigene: 380271
NBT02251