|Catalog No||Formulation||Size||Price (USD)|
|2810-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|2810-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||429.00|
|2810-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||429.00|
|Human Entrez Gene ID||2810|
|Human Gene Symbol||SFN|
|Human Chromosome Location||1p36.11|
|Synonyms||SFN; HME1; Stratifin; YWHAS; 14-3-3 Sigma|
|Immunogen||Recombinant human full-length SFNprotein|
|Host / Ig Isotype||Mouse / IgG1, kappa|
|Mol. Weight of Antigen||28kDa|
|Cellular Localization||Cytoplasm and nucleus. May be secreted by a non-classical secretory pathway.|
|Species Reactivity||Human. Others-not tested.|
SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.
Western Blotting (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.