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Home >> Antibodies >> 14-3-3 Sigma / Stratifin

14-3-3 Sigma / Stratifin

Mouse Monoclonal Antibody [Clone CPTC-SFN-2]

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Catalog No Formulation Size Price (USD)  
2810-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
2810-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
2810-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 2810
Human SwissProt P31947
Human Unigene 523718
Human Gene Symbol SFN
Human Chromosome Location 1p36.11
Synonyms SFN; HME1; Stratifin; YWHAS; 14-3-3 Sigma
Immunogen Recombinant human full-length SFNprotein
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 28kDa
Cellular Localization Cytoplasm and nucleus. May be secreted by a non-classical secretory pathway.
Species Reactivity Human. Others-not tested.

Specificity & Comments

SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Known Applications & Suggested Dilutions

Western Blotting (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Pulukuri, S M, and J S Rao. Oncogene., U.S. National Library of Medicine, 3 Aug. 200
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