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Home >> Antibodies >> 14-3-3 Sigma / Stratifin

14-3-3 Sigma / Stratifin

Mouse Monoclonal Antibody [Clone CPTC-SFN-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Skin stained with Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).

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SDS-PAGE Analysis Purified Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of Human PC-3 cell lysate using Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 Sigma / Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).

Product Details

Synonyms

SFN; HME1; Stratifin; YWHAS; 14-3-3 Sigma

Positive Control

PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length SFN protein

Cellular Localization

Cytoplasm and nucleus. May be secreted by a non-classical secretory pathway.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

28kDa

Specificity & Comments

SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Pulukuri, S M, and J S Rao. Oncogene., U.S. National Library of Medicine, 3 Aug. 2006.

Bioinformatics

  •  Entrez Gene ID: 2810
  •  Gene Symbol: SFN
  •  SwissProt: P31947
  •  Chromosome Location: 1p36.11
  •  Unigene: 523718
NBT02481