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Cystatin A Antibody [MSVA-461M]

In Stock
Catalog Number Formulation Size Price
1475-MSM10-P0
Purified Ab with BSA and Azide
20ug
$249.00
1475-MSM10-P1
Purified Ab with BSA and Azide
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CSTA Antibody in Numerous Tissues (IHC)

CSTA Antibody in Numerous Tissues (IHC)

Cystatin-A Mouse Monoclonal Antibody (MSVA-461M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology, Nuclear Marker
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cytoplasm
Gene Name
CSTA
Positive Control
Skin: A strong nuclear and cytoplasmic Cystatin A immunostaining should be seen in the granular layer.
Immunogen
Recombinant full-length human CSTA protein
Alternate Names
Cystatin-A, Cystatin-AS, Stefin-A, STF1; STFA; AREI; cstA; Stefin A

Database Links

Entrez Gene ID
1475
SwissProt
P01040

Additional Information

Clone
MSVA-461M
Chromosome Location
3Q21.1
Mol. Weight of Antigen
11kDa
Expression System
CHO Cells

Related Antibodies

View All CSTA Antibodies

Functions

  • This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

Key References

  • Takahashi, H., et al. 2001. J. Biol. Chem. 276: 36632-36638

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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