Cystatin A [CSTA/2882]

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Summary

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cytoplasm
Light Chain
Gene Name
Positive Control
Human tonsil or prostate tissue (IHC).
Immunogen
Recombinant fragment of human CSTA protein (around aa 1-98) (exact sequence is proprietary)
Alternate Names
Cystatin-A, Cystatin-AS, Stefin-A, STF1; STFA; AREI; cstA; Stefin A

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
CSTA/2882
Human Gene Symbol
CSTA
Chromosome Location
3Q21.1
Mol. Weight of Antigen
11kDa

Functions

  • This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Takahashi, H., et al. 2001. J. Biol. Chem. 276: 36632-36638.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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