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Aldo-keto Reductase Family 1 Member C2 / DD2 Antibody [CPTC-AKR1C2-1]

In Stock
Catalog Number Formulation Size Price
1646-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
1646-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
1646-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Aldo-keto Reductase Family 1 Member C2 / DD2 Antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AKR1C2 Mouse Monoclonal Antibody (CPTC-AKR1C2-1).

Aldo-keto Reductase Family 1 Member C2 / DD2 Antibody - Image 2

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AKR1C2 Mouse Monoclonal Antibody (CPTC-AKR1C2-1).

Aldo-keto Reductase Family 1 Member C2 / DD2 Antibody - Image 3

Western Blot Analysis of Human HeLa, K-562 and A431 cell lysates using AKR1C2 Mouse Monoclonal Antibody (CPTC-AKR1C2-1).

Aldo-keto Reductase Family 1 Member C2 / DD2 Antibody - Image 4

SDS-PAGE Analysis Purified AKR1C2 Mouse Monoclonal Antibody (CPTC-AKR1C2-1). Confirmation of Purity and Integrity of Antibody.

AKR1C2 Antibody in Western Blot (WB)

AKR1C2 Antibody in Western Blot (WB)

Western blot analysis of (1) human liver, (2) mouse liver, (3) rat liver, (4) hamster liver, (5) guinea pig liver and (6) HepG2 cell lysates using AKR1C2 Mouse Monoclonal Antibody (CPTC-AKR1C2-1).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

DDH2 / AKR1C2 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Nuclear Marker
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Cytosol
Gene Name
AKR1C2
Positive Control
A431, A549 cells. Human liver or stomach tissue., HeLa, Hep G2, K-562
Immunogen
Recombinant human full-length AKR1C2 protein
Alternate Names
Aldo-keto reductase family 1 member C2, 3-alpha-HSD3, Chlordecone reductase homolog HAKRD, Dihydrodiol dehydrogenase 2, Dihydrodiol dehydrogenase/bile acid-binding protein, Type III 3-alpha-hydroxysteroid dehydrogenase, AKR1C2, 3-alpha-HSD3, AKR1C-pseudo protein, BABP, Dihydrodiol dehydrogenase 2, DD-2, MCDR2, HAKRD, DD, DDH2, HBAB, Pseudo-chlordecone reductase, SRXY8

Database Links

Entrez Gene ID
1646
SwissProt
P52895

Additional Information

Clone
CPTC-AKR1C2-1
Chromosome Location
10p15.1
Mol. Weight of Antigen
37kDa

Related Antibodies

View All AKR1C2 Antibodies

Functions

  • Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol) (PubMed:15929998, PubMed:17034817, PubMed:17442338, PubMed:8573067). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT (PubMed:10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699).

Key References

  • Binstock, J.M., et al. 1992. Res. Commun. 187: 760-766

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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