Printable Datasheet

Recombinant Tyrosinase (Melanoma Marker) Antibody [rOCA1/812]

In Stock
Catalog Number Formulation Size Price
7299-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7299-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7299-MSM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Tyrosinase (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded Melanoma stained with Tyrosinase MouseRecombinant Monoclonal Antibody (rOCA1/812).

TYR Antibody in SDS-PAGE

TYR Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Tyrosinase Recombinant Mouse Monoclonal Antibody (rOCA1/812). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants.This MAb is a useful marker for melanocytes and melanomas.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Melanosome, Melanosome membrane
Gene Name
TYR
Positive Control
SK-MEL-13, SK-MEL-19, SK-MEL-30 or SK-MEL-37 cells. Human melanoma.
Immunogen
Recombinant full-length human Tyrosinase protein
Alternate Names
Tyrosinase, ATN|CMM8|OCA1|OCA1A|OCAIA|SHEP3

Database Links

Entrez Gene ID
7299
SwissProt
P14679

Additional Information

Clone
rOCA1/812
Chromosome Location
11q14.3
Mol. Weight of Antigen
60.39kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All TYR Antibodies

Functions

  • This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:28661582).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales