PARTNERSHIP OPPORTUNITIES
SDS-PAGE Analysis of Purified LIN28A Mouse Monoclonal Antibody (PCRP-LIN28A-1E2). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of PFA-fixed HeLa cells. LIN28A Mouse Monoclonal Antibody (PCRP-LIN28A-1E2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using LIN28A Mouse Monoclonal Antibody (PCRP-LIN28A-1E2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II.
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