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Ku (p70/p80) (Nuclear Marker) Antibody [KU729]

Name: Ku (p70/p80) (Nuclear Marker) Antibody
SKU: 2547-MSM1-PE-100T
Availability: InStock

In Stock

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Ku (p70/p80) Antibody in Flow Cytometry (FC).

Flow Cytometric analysis of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku Monoclonal Antibody (KU729).

Ku (p70/p80) Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Ku Mouse Monoclonal Antibody (KU729). Confirmation of Purity and Integrity of Antibody.

Ku (p70/p80) Antibody in Flow Cytometry (FC)

Flow Cytometry of Human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled Ku Monoclonal Antibody (KU729).

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Summary

Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence

Species Reactivity

Human

Research Areas

Immunology, Infectious Disease

Isotype / Light Chain

IgG1 /

Cellular Localization

Chromosome, Nucleus

Gene Name

XRCC6

Positive Control

Human cancer K562

Immunogen

Nuclear extract of human HL-60 cells

Alternate Names

X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hamster cells 6, 70kDa / 80kDa subunit of Ku antigen; CTC box-binding factor 75kDa subunit; CTC75; CTCBF; G22P1; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6); ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; CTCBF; KARP1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); X-ray repair cross-complementing protein 5 (XRCC5)

Database Links

Entrez Gene ID

2547 (p70); 7520 (p80)

SwissProt

P12956

Additional Information

Clone

KU729

Chromosome Location

22q13.2 (p70); 2q35 (p80)

Mol. Weight of Antigen

70kDa & 80kDa

Functions

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Required for double-strand break repair and V(D)J recombination (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Also has a role in chromosome translocation (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Has a role in chromosome translocation (PubMed:7957065, PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:8621488, PubMed:12145306, PubMed:11493912). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed:7957065, PubMed:8621488, PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:12145306, PubMed:11493912). It works in the 3'-5' direction (PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed:7957065, PubMed:8621488, PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:12145306, PubMed:11493912). Binding to DNA may be mediated by XRCC6 (PubMed:20493174, PubMed:2466842, PubMed:9742108, PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912). The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912, PubMed:20493174, PubMed:2466842, PubMed:9742108). Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed:20383123). 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed:20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:12145306). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).

Key References

Walker, J. R., et al. 2001. Nature 412:607.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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