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Recombinant CD45 / LCA (B-Cell Marker) Antibody [PTPRC/1975R]

In Stock
Catalog Number Formulation Size Price
5788-RBM25-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
5788-RBM25-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
5788-RBM25-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD45 / LCA (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD45 RabbitRecombinant Monoclonal Antibody (PTPRC/1975R).

Recombinant CD45 / LCA (B-Cell Marker) Antibody - Image 2

Western Blot Analysis of Human spleen tissue lysate using CD45 Rabbit Recombinant Monoclonal Antibody (PTPRC/1975R).

Recombinant CD45 / LCA (B-Cell Marker) Antibody - Image 3

Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using CD45 Rabbit Recombinant Monoclonal Antibody (PTPRC/1975R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Recombinant CD45 / LCA (B-Cell Marker) Antibody - Image 4

Paraformaldehyde-fixed Raji cells stained with CD45 Rabbit Recombinant Monoclonal Antibody (PTPRC/1975R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).

PTPRC Antibody in SDS-PAGE

PTPRC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Receptor-type tyrosine-protein phosphatase C Recombinant Rabbit Monoclonal Antibody (PTPRC/1975R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Autophagy, Cancer, Developmental Biology, Immuno Oncology, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Membrane raft
Gene Name
PTPRC
Positive Control
Ramos, U-698 or GA-10 cells. Tonsil and Spleen.
Immunogen
Human recombinant full-length CD45 protein
Alternate Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Database Links

Entrez Gene ID
5788
SwissProt
P08575

Additional Information

Clone
PTPRC/1975R
Chromosome Location
1q31.3
Mol. Weight of Antigen
180-220kDa

Related Antibodies

View All PTPRC Antibodies

Functions

  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
  • (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

Key References

  • Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.
  • Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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