Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

RAD51 (Prognostic and Response to Chemotherapy Marker) Antibody [RAD51/2753]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
5888-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5888-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5888-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Western Blot (WB)
2-4ug/ml

Summary

RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies.RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG /
Cellular Localization
Centrosome, Chromosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Mitochondrion matrix, Nucleus, Perinuclear region
Gene Name
Positive Control
HeLa or MCF-7 cells. Colon or breast carcinoma.
Immunogen
Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)
Alternate Names
DNA repair protein RAD51 homolog 1, RAD51 homolog A, RCA1/BRCA2 containing complex, subunit 5; BRCC5; DNA repair protein RAD51; FANCR; HsT16930; MRMV2; RAD51; RAD51 recombinase; RAD51A; RECA; RecA like protein; Recombination protein A

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
RAD51/2753
Chromosome Location
15q15.1
Mol. Weight of Antigen
37kDa

Functions

  • Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).

Key References

  • Henning W, Stuerzbecher H-W (2003) Homologous recombination and cell cycle checkpoints: Rad51 in tumour progression and therapy resistance. Toxicology 193: 91-109

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “RAD51 (Prognostic and Response to Chemotherapy Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK