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Home >> Antibodies >> PRMT7

PRMT7

Mouse Monoclonal Antibody [Clone PCRP-PRMT7-1A7]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. PRMT7 Mouse Monoclonal Antibody (PCRP-PRMT7-1A7) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PRMT7 Mouse Monoclonal Antibody (PCRP-PRMT7-1A7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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SDS-PAGE Analysis. Purified PRMT7 Mouse Monoclonal Antibody (PCRP-PRMT7-1A7). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PRMT7 Mouse Monoclonal Antibody (PCRP-PRMT7-1A7).

Product Details

Synonyms

[Myelin basic protein]-arginine N-methyltransferase PRMT7; Histone-arginine N-methyltransferase PRMT7; KIAA1933; Protein arginine methyltransferase 7; Protein arginine N-methyltransferase 7

Positive Control

HeLa, MCF7 or HepG2 cells.

Known Applications & Suggested Dilutions

IP, Western Blot, IF, Flow Cyt         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human PRMT7 protein

Cellular Localization

Nucleus. Nucleoplasm. Cytoplasm.

Species Reactivity

Human. Predicted to react with Rat.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

78.46kDa

Specificity & Comments

Arginine methylation is an irreversible protein modification catalyzed by Arginine methyltransferases, such as PRMT7, which uses S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport and RNA splicing. PRMT7 has two methyltransferase domains, each containing a putative AdoMet-binding motif. The N-terminal methyltransferase domain closely resembles the catalytic core of PRMT5, and the C-terminal domain is most similar to that of PRMT1. Three PRMT7 splice variants have been identified by database analysis. PRMT7 is localized to the nucleus and cytoplasm and moderate expression is observed in adult brain and lung tissues.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 54496
  •  Gene Symbol: PRMT7
  •  SwissProt: Q9NVM4
  •  Chromosome Location: 16q22.1
  •  Unigene: 640229