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Home >> Antibodies >> Neurofilament (H+L) (Neuronal Marker)

Neurofilament (H+L) (Neuronal Marker)

Mouse Monoclonal Antibody [Clone RT-97 + NR-4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).

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Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).

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Flow Cytometric Analysis of HEK293 cells using Neurofilament Monoclonal Antibody (RT-97 + NR-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Product Details

Synonyms

NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200. 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68

Positive Control

HEK293 cells; Cerebellum, Neuroblastoma.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Triton-X 100 insoluble proteins of rat brain (RT-97); Neurofilaments from porcine spinal cord (NR-4)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Chicken. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

200kDa & 68kDa

Specificity & Comments

This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ishii M et. al. Fukushima J Med Sci. 2004;50(2):65-74.
  2. Angelides, K.J., et. al. 1989. J. Cell Biol. 108: 1495-1506.

Bioinformatics

  •  Entrez Gene ID: 4744
  •  Gene Symbol: NEFH & NEFL
  •  SwissProt: P12036
  •  Chromosome Location: 22q12.2 (NEFH) & 8p21.2 (NEFL)
  •  Unigene: 198760
NBT01201