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Home >> Antibodies >> Neurofilament (NF-H) (Neuronal Marker)

Neurofilament (NF-H) (Neuronal Marker)

Recombinant Mouse Monoclonal Antibody [Clone rNF421]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Brain stained with Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).

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SDS-PAGE Analysis Purified Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human brain tissue lysate using Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421).

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Flow Cytometric Analysis of HEK293 cells using Neurofilament Mouse Recombinant Monoclonal Antibody (rNF421) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Product Details

Synonyms

NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200

Positive Control

HEK293 cells, Brain, Neuroblastoma.

Known Applications & Suggested Dilutions

Western Blot, Flow Cyt, IHC-P         More Details

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human NEFH protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Pig. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

200kDa

Specificity & Comments

This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Shaw G et. al. European Journal of Cell Biology. 42: 1-9 (1986).2. Shaw G et. al. European Journal of Cell Biology. 34: 130-136 (198

Bioinformatics

  •  Entrez Gene ID: 4744
  •  Gene Symbol: NEFH
  •  SwissProt: P12036
  •  Chromosome Location: 22q12.2
  •  Unigene: 19876
NBT02348