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Home >> Antibodies >> Myogenin / Myf-4 (Skeletal Muscle Marker) (Transcription Factor)

Myogenin / Myf-4 (Skeletal Muscle Marker) (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-MYOG-1C5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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SDS-PAGE Analysis Purified Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Myogenin Mouse Monoclonal Antibody (PCRP-MYOG-1C5).

Product Details

Synonyms

bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Positive Control

Rh-30 or HeLa cells. Human skeletal muscle or rhabdomyosarcoma.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Human myogenin recombinant protein

Cellular Localization

Nucleus.

Species Reactivity

Human, Mouse andRat.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

34kDa

Specificity & Comments

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wang NP et. al. Am J Pathol 1995, 147:1799-1810.

Bioinformatics

  •  Entrez Gene ID: 4656
  •  Gene Symbol: MYOG
  •  SwissProt: P15173
  •  Chromosome Location: 1q32.1
  •  Unigene: 2830