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Home >> Antibodies >> MLX

MLX

Mouse Monoclonal Antibody [Clone PCRP-MLX-1G8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti-mouse IgG-CF488 (green); counterstain phalloidin (red).

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SDS-PAGE Analysis Purified MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8).

Product Details

Synonyms

bHLHd13; Class D basic helix-loop-helix protein 13; MAD7; Max-like bHLHZip protein; Max-like protein X; MLXIP; MONDOA; MXD7; Protein BigMax; Protein Mlx; Transcription factor-like protein 4 (TCFL4)

Positive Control

HeLa or MCF7 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MLX protein

Cellular Localization

Nucleus and cytoplasm.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

30kDa

Specificity & Comments

Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad1, Mad3, Mad4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad1, Mad4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackwood, E.M., et al. 1991. Science 251:1211-1217.
  2. Amati, B., et al. 1992. Cell 72: 233-245.

Bioinformatics