Flow Cytometric Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti-mouse IgG-CF488 (green); counterstain phalloidin (red).
SDS-PAGE Analysis Purified MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad1, Mad3, Mad4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad1, Mad4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.