2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> MED7

MED7

Mouse Monoclonal Antibody [Clone PCRP-MED7-1B8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

SDS-PAGE Analysis Purified MED7 Mouse Monoclonal Antibody (PCRP-MED7-1B8). Confirmation of Purity and Integrity of Antibody.

img

Flow cytometric analysis of PFA-fixed HeLa cells. MED7 Mouse Monoclonal Antibody (PCRP-MED7-1B8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED7 Mouse Monoclonal Antibody (PCRP-MED7-1B8) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using MED7-Monospecific Mouse Monoclonal Antibody (PCRP-MED7-1B8).

Product Details

Synonyms

Activator recruited cofactor 34 kDa component; ARC34; Cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa; CRSP complex subunit 9; CRSP9; hMED7; Mediator of RNA polymerase II transcription subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; Transcriptional coactivator CRSP33

Positive Control

HeLa, U-251 MG or CACO-2 cells.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IP         More Details

ELISA (use assay dependent concentration)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MED7 protein

Cellular Localization

Nucleus. Nuclear bodies.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

27.2kDa

Specificity & Comments

MED7, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 9443
  •  Gene Symbol: MED7
  •  SwissProt: O43513
  •  Chromosome Location: 6q26
  •  Unigene: 279902