2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> MED22 (Transcription Factor)

MED22 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-MED22-2A7]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

SDS-PAGE Analysis Purified MED22 Mouse Monoclonal Antibody (PCRP-MED22-2A7). Confirmation of Purity and Integrity of Antibody.

img

Flow cytometric analysis of PFA-fixed U87 cells. MED22 Mouse Monoclonal Antibody (PCRP-MED22-2A7) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

img

Immunofluorescence Analysis of PFA-fixed U87 cells stained using MED22 Mouse Monoclonal Antibody (PCRP-MED22-2A7) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using MED22 Mouse Monoclonal Antibody (PCRP-MED22-2A7).

Product Details

Synonyms

Med24; SURF5; mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22

Positive Control

HeLa or U87 cells.

Known Applications & Suggested Dilutions

ELISA, IF, IP, Flow Cyt         More Details

ELISA (For coating, order antibody without BSA)
Immunofluorescence (0.5-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (0.5-2ug/million cells)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MED22 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

22kDa

Specificity & Comments

Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 6837
  •  Gene Symbol: MED22
  •  SwissProt: Q15528
  •  Chromosome Location: 9q34.2
  •  Unigene: 78354