SDS-PAGE Analysis Purified MED22 Mouse Monoclonal Antibody (PCRP-MED22-1E4). Confirmation of Purity and Integrity of Antibody.
Flow cytometric analysis of PFA-fixed HeLa cells. MED22 Mouse Monoclonal Antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using MED22 Mouse Monoclonal Antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Analysis of Protein Array containing more than 19,000 full-length human proteins using MED22 Mouse Monoclonal Antibody (PCRP-MED22-1E4).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.