2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> MCAM (Melanoma Cell Adhesion Molecule) / MUC18 / CD146

MCAM (Melanoma Cell Adhesion Molecule) / MUC18 / CD146

Mouse Monoclonal Antibody [Clone SPM620]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Melanoma stained with MCAM Monoclonal Antibody (SPM620)

Product Details

Synonyms

Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1

Positive Control

A-375, HUV-EC or HeLa cells. Tonsil or Melanoma (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human MCAM protein

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

130kDa

Specificity & Comments

The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Pruszak J et al. Stem Cells 25:2257-68 (2007)

Bioinformatics

  •  Entrez Gene ID: 4162
  •  Gene Symbol: MCAM
  •  SwissProt: P43121
  •  Chromosome Location: 11q23.3
  •  Unigene: 599039
NBT01101