2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> MBNL3

MBNL3

Mouse Monoclonal Antibody [Clone PCRP-MBNL3-1D11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Flow Cytometric Analysis of PFA-fixed HeLa cells. MBNL3 Mouse Monoclonal Antibody (PCRP-MBNL3-1D11) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

img

SDS-PAGE Analysis Purified MBNL3 Mouse Monoclonal Antibody (PCRP-MBNL3-1D11). Confirmation of Purity and Integrity of Antibody.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using MBNL3 Mouse Monoclonal Antibody (PCRP-MBNL3-1D11).

Product Details

Synonyms

CHCR; Cys3His CCG1 required protein; MBLX; MBLX39; Muscleblind like protein 3; Muscleblind like splicing regulator 3; Muscleblind-like X-linked protein; Protein HCHCR; RP23-98P13.1

Positive Control

HeLa or MCF7 cells.

Known Applications & Suggested Dilutions

Flow Cyt         More Details

Flow Cytometry (1-2ug/million cells)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MBNL3 protein

Cellular Localization

Nucleus. Cytoplasm. Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

39kDa

Specificity & Comments

Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and 2, which associate with expanded CUG repeats in vitro, localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating MBNL1 and 2 in the pathogenesis of both disorders. MBNL3, a 354 amino acid protein, inhibits expression of muscle differentiation, opposite to the function of MBNL1, which functions as a promoter of muscle differentiation. MBNL3 shows strong expression in placenta.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ishikawa, K., et al. 1998. DNA Res. 4: 307-313.
  2. Ishiura, S. 2005. Molecular pathways to myotonic dystrophy. Nippon Rinsho 63: 515-521.

Bioinformatics