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Home >> Antibodies >> MAPK1 / ERK2 (Transcription Factor)

MAPK1 / ERK2 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-MAPK1-1D1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified MAPK1 Mouse Monoclonal Antibody (PCRP-MAPK1-1D1). Confirmation of Purity and Integrity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. MAPK1 Mouse Monoclonal Antibody (PCRP-MAPK1-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingMAPK1 Mouse Monoclonal Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using MAPK1 Mouse Monoclonal Antibody (PCRP-MAPK1-1D1).

Product Details

Synonyms

Extracellular Signal Regulated Kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; Mapk1; MAPK2; P38; P40; P41; p42-MAPK; PRKM1; PRKM2; protein kinase, mitogen-activated, 1 / 2; protein tyrosine kinase ERK2; ERT1

Positive Control

HeLa or U87 cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (0.5-2ug/million cells)
Immunofluorescence (0.5-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MAPK1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

41.3kDa

Specificity & Comments

Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 5594
  •  Gene Symbol: MAPK1
  •  SwissProt: P28482
  •  Chromosome Location: 22q11.2
  •  Unigene: 431850