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Home >> Antibodies >> IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker)

IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker)

Mouse Monoclonal Antibody [Clone IM373]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to CF488

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to CF488

Price (USD)

429.00
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SDS-PAGE Analysis Purified IgM Mouse Monoclonal Antibody (IM373). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region

Positive Control

293T, Raji or hPBL cells. Tonsil or Spleen.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length heavy chain of human IgM

Cellular Localization

Cytoplasm, Cell Surface and Secreted

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

50-75kDa

Specificity & Comments

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Baryshnikov AIu. Gematol Transfuziol. 1990;35(8):4-7.

Bioinformatics

  •  Entrez Gene ID: 3507
  •  Gene Symbol: IGHM
  •  SwissProt: P01871, P20769
  •  Chromosome Location: 14q32.33
  •  Unigene: 510635
NBT00373