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Home >> Antibodies >> ID1 (Inhibitor of DNA-binding) (Transcription Factor)

ID1 (Inhibitor of DNA-binding) (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-ID1-2F11]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. ID1 Mouse Monoclonal Antibody (PCRP-ID1-2F11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ID1 Mouse Monoclonal Antibody (PCRP-ID1-2F11). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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SDS-PAGE Analysis Purified ID1 Mouse Monoclonal Antibody (PCRP-ID1-2F11). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using ID1-Monospecific Mouse Monoclonal Antibody (PCRP-ID1-2F11).

Product Details

Synonyms

bHLHb24; Class B basic helix-loop-helix protein 24; DNA binding protein inhibitor ID1; Dominant negative helix loop helix protein; Inhibitor of Differentiation 1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Positive Control

HeLa or HepG2 cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (aa 5-150) of human ID1

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

16.13kDa

Specificity & Comments

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 3397
  •  Gene Symbol: ID1
  •  SwissProt: P41134
  •  Chromosome Location: 20q11.21
  •  Unigene: 504609