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HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rHSPD1/6495]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA or Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human thyroid stained with HSP60Recombinant Mouse Monoclonal Antibody (rHSPD1/6495).

SDS-PAGE Analysis Purified HSP60Recombinant Mouse Monoclonal Antibody (rHSPD1/6495). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Hep2 cell lysate using HSP60Recombinant Mouse Monoclonal Antibody (rHSPD1/6495).

Formalin-fixed, paraffin-embedded human thyroid stained with HSP60Recombinant Mouse Monoclonal Antibody (rHSPD1/6495).

SDS-PAGE Analysis Purified HSP60Recombinant Mouse Monoclonal Antibody (rHSPD1/6495). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Hep2 cell lysate using HSP60Recombinant Mouse Monoclonal Antibody (rHSPD1/6495).

Product Details

Synonyms

Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)

Positive Control

HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HSPD1 protein

Cellular Localization

Mitochondria in cytoplasm

Species Reactivity

Human, Monkey, Mouse, Rat, Hamster, Guinea pig andChicken.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.