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Home >> Antibodies >> Histone Deacetylase 1 (HDAC1)

Histone Deacetylase 1 (HDAC1)

Mouse Monoclonal Antibody [Clone PCRP-HDAC1-1B7]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 Mouse Monoclonal Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis Purified HDAC1 Mouse Monoclonal Antibody (PCRP-HDAC1-1B7). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Mouse Monoclonal Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1-Monospecific Mouse Monoclonal Antibody (PCRP-HDAC1-1B7).

Product Details

Synonyms

DKFZp686H12203; GON 10; HD1; Reduced potassium dependency yeast homolog like 1 (RPD3L1); RPD3

Positive Control

HeLa or Jurkat cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HDAC1 protein

Cellular Localization

Nucleus. Nucleoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG

Mol. Weight of Antigen

60kDa

Specificity & Comments

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/ CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 3065
  •  Gene Symbol: HDAC1
  •  SwissProt: Q13547
  •  Chromosome Location: 1p35.1
  •  Unigene: 88556