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Home >> Antibodies >> HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)

HIF1 alpha (Hypoxia-Inducible Factor 1-alpha)

Mouse Monoclonal Antibody [Clone ESEE122]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified HIF1 alpha Mouse Monoclonal Antibody (ESEE122). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS superfamily 1 (MOP1); PAS domain-containing protein 8 (PASD8)

Positive Control

Cobalt chloride treated HeLa cells. Colon, Renal, Breast or Bladder Carcinomas.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF         More Details

ELISA (For coating, purchase antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

GST-human HIF-1A amino acids 329-530 fusion protein

Cellular Localization

Nuclear and cytoplasmic

Species Reactivity

Human. Cow. Dog. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

92-110kDa

Specificity & Comments

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wiesener et al. 1998. Blood. 92(7):2260-8.
  2. Talks et al. 2000. Am J Pathol. 157(2):411-21.

Bioinformatics

  •  Entrez Gene ID: 3091
  •  Gene Symbol: HIF1A
  •  SwissProt: Q16665
  •  Chromosome Location: 14q23.2
  •  Unigene: 597216
NBT01574