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Home >> Antibodies >> GTF2B/ TFIIB (Transcription Factor 2B)

GTF2B/ TFIIB (Transcription Factor 2B)

Mouse Monoclonal Antibody [Clone PCRP-GTF2B-1D1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. GTF2BMouse Monoclonal Antibody (PCRP-GTF2B-1D1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis Purified GTF2B Mouse Monoclonal Antibody (PCRP-GTF2B-1D1). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2B-Monospecific Mouse Monoclonal Antibody (PCRP-GTF2B-1D1).

Product Details

Synonyms

General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TFIIB; Transcription initiation factor IIB

Positive Control

HeLa or MCF-7 cells.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IP         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human GTF2Bprotein

Cellular Localization

Nucleus. Nucleoplasm, nuclear bodies.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

34.8kDa

Specificity & Comments

In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 2959
  •  Gene Symbol: GTF2B
  •  SwissProt: Q00403
  •  Chromosome Location: 1p22.2
  •  Unigene: 481852