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Home >> Antibodies >> Glycophorin A / CD235a (Erythrocyte Marker)

Glycophorin A / CD235a (Erythrocyte Marker)

Recombinant Mouse Monoclonal Antibody [Clone rGYPA/280]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Glycophorin A Mouse Recombinant Monoclonal Antibody (rGYPA/280).

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SDS-PAGE Analysis Purified Glycophorin A Mouse Recombinant Monoclonal Ab (rGYPA/280). Confirmation of Purity and Integrity of Antibody

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Western Blot Analysis of K562 cell lysate usingGlycophorin A Mouse Recombinant Monoclonal Antibody (rGYPA/280).

Product Details

Synonyms

Blood group--MN locus; GPA; GPErik; GpMiIII; GPSAT; GYPA; MN sialoglycoprotein; MNS; PAS2; Sialoglycoprotein alpha

Positive Control

Erythrocytes in any human tissues. K562 cell lysate.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human glycophorin A protein

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

39kDa

Specificity & Comments

Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Andersson, L.C., et al. 1979. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia. Int. J. Cancer 23: 717-720.
  2. Liszka, K., et al., 1983. Glycophorin A expression in malignant hematopoiesis. Am. J. Hematol. 15: 219-226.

Bioinformatics

NBT01868