Formalin-fixed, paraffin-embedded human Liver stained with Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).
SDS-PAGE Analysis Purified Cytochrome C Recombinant Rabbit Monoclonal (CYCS/3128R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human heart tissue lysate using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).
Flow Cytometric Analysis of PFA fixed HeLa cells using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by goat anti-rabbit IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 is Control (Red).
Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by goat anti-rabbit IgG-CF488 (Green)
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).