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Home >> Antibodies >> Cytochrome C (Mitochondrial Marker)

Cytochrome C (Mitochondrial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone CYCS/3128R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Liver stained with Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).

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SDS-PAGE Analysis Purified Cytochrome C Recombinant Rabbit Monoclonal (CYCS/3128R). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human heart tissue lysate using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R).

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Flow Cytometric Analysis of PFA fixed HeLa cells using Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by goat anti-rabbit IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 is Control (Red).

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Immunofluorescence Analysis of PFA fixed HeLa cells labeling Cytochrome C Recombinant Rabbit Monoclonal Antibody (CYCS/3128R) followed by goat anti-rabbit IgG-CF488 (Green)

Product Details

Synonyms

CYC; CYCS; HCS; THC4

Positive Control

K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle. Human heart lysate.

Known Applications & Suggested Dilutions

IF, Western Blot, Flow Cyt, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CYCS protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Horse. Dog. Pigeon. Frog. Drosophila.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

15kDa

Specificity & Comments

It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Goshorn SG., et al. J Biol Chem 266:2134-2142 (1991).

Bioinformatics

  •  Entrez Gene ID: 54205
  •  Gene Symbol: CYCS
  •  SwissProt: P99999
  •  Chromosome Location: 7p15.3
  •  Unigene: 437060
NBT03128