Home >> Antibodies >> CD63 (Late Endosomes Marker)

CD63 (Late Endosomes Marker)

Mouse Monoclonal Antibody [Clone SPM524]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (SPM524) (AEC chromogen).

Formalin-fixed, paraffin-embedded human prostate stained with CD63 Mouse Monoclonal Antibody (SPM524) (DAB chromogen).

Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (SPM524) (AEC chromogen).

Formalin-fixed, paraffin-embedded human prostate stained with CD63 Mouse Monoclonal Antibody (SPM524) (DAB chromogen).

Product Details

Synonyms

gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; Melanoma associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30

Positive Control

HL60, THP-1 or NIH/3T3 cells. Human melanoma or lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Smooth plasma membrane fraction of MeWo cells

Cellular Localization

Cell Surface. Cytoplasm.

Species Reactivity

Human and mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

26kDa (core protein); 30-60kDa (glycosylated)

Specificity & Comments

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.