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Home >> Antibodies >> CD6 (Negative Marker of T-regulatory Cells)

CD6 (Negative Marker of T-regulatory Cells)

Mouse Monoclonal Antibody [Clone C6/372+3F7B5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Monoclonal Antibody (C6/372+3F7B5).

img

Immunofluorescence staining of MOLT-4 cells using CD6 Monoclonal Antibody (C6/372+3F7B5) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Product Details

Synonyms

T12; TP120

Positive Control

MOLT-4, CCRF-CEM, Jurkat cells, Tonsil.

Known Applications & Suggested Dilutions

IF, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human recombinant CD6 protein (C6/372); Human rheumatoid synovial T cell line ST-1 (3F7B5)

Cellular Localization

Cell surface and Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

90-130kDa

Specificity & Comments

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bazil, V et. al. 1989. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia. Biol. 35:289-297.

Bioinformatics

  •  Entrez Gene ID: 923
  •  Gene Symbol: CD6
  •  SwissProt: P30203
  •  Chromosome Location: 11q12.2
  •  Unigene: 744366
NBT01228