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CD6

Mouse Monoclonal Antibody [Clone C6/372]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with CD6 Mouse Monoclonal Antibody (C6/372).

SDS-PAGE Analysis Purified CD6 Mouse Monoclonal Antibody (C6/372). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence analysis of PFA-fixed MOLT-4 cells. CD6 Mouse Monoclonal Antibody (C6/372) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.

Formalin-fixed, paraffin-embedded human tonsil stained with CD6 Mouse Monoclonal Antibody (C6/372).

SDS-PAGE Analysis Purified CD6 Mouse Monoclonal Antibody (C6/372). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence analysis of PFA-fixed MOLT-4 cells. CD6 Mouse Monoclonal Antibody (C6/372) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.

Product Details

Synonyms

T12; TP120

Positive Control

MOLT-4, CCRF-CEM or Jurkat cells. Human tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human recombinant CD6 protein

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

90-130kDa

Specificity & Comments

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.