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Home >> Antibodies >> CD45RB (B-Cell Marker)

CD45RB (B-Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone PTPRC/1783R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed paraffin-embedded human tonsil stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R).

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Immunofluorescent analysis of PFA-fixed Raji cells. CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (green). Counterstain is phalloidin (red).

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Flow Cytometric Analysis of PFA-fixed Raji cells. CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).

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Western Blot Analysis of human spleen tissue lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783).

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SDS-PAGE Analysis Purified CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

Ramos, U-698, or GA-10 cells. Human tonsil or spleen.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CD45RB protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

180-220kDa

Specificity & Comments

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Related Products



200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.
  2. Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62.

Bioinformatics

  •  Entrez Gene ID: 5788
  •  Gene Symbol: PTPRC
  •  SwissProt: P08575
  •  Chromosome Location: 1q31.3
  •  Unigene: 654514