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Home >> Antibodies >> CD45 / LCA (Leucocyte Marker)

CD45 / LCA (Leucocyte Marker)

Mouse Monoclonal Antibody [Clone 2B11 + PD7/26]

Catalog #

Size

0.5 ml at 100ug/ml

Price (USD)

429.00

Formulation

Purified Ab conjugated to FITC

Catalog No

Size

0.5 ml at 100ug/ml

Formulation

Purified Ab conjugated to FITC

Price (USD)

429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).

Product Details

Synonyms

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

Ramos, U-698 or GA-10 cells. Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Isolated neoplastic cells from T cell lymphoma (2B11); and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human. Dog.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

180-220kDa

Specificity & Comments

Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.

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Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

Key References

  1. Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.
  2. Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62.

Bioinformatics

  •  Entrez Gene ID: 5788
  •  Gene Symbol: PTPRC
  •  SwissProt: P08575
  •  Chromosome Location: 1q31.3
  •  Unigene: 654514
NBT00745