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Home >> Antibodies >> CD13 / Aminopeptidase-N (Myeloid Cell Marker)

CD13 / Aminopeptidase-N (Myeloid Cell Marker)

Mouse Monoclonal Antibody [Clone APN/6998]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Western blot analysis of human kidney tissue lysate using CD13 Mouse Monoclonal Antibody (APN/6998).

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Formalin-fixed, paraffin-embedded human tonsil stained with CD13 Mouse Monoclonal Antibody (APN/6998). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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Formalin-fixed, paraffin-embedded human kidney stained with CD13 Mouse Monoclonal Antibody (APN/6998). Inset: PBS instead of primary antibody; secondary only negative control.

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Formalin-fixed, paraffin-embedded human prostate stained with CD13 Mouse Monoclonal Antibody (APN/6998). Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Synonyms

Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; gp150; hAPN; Lap1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN

Positive Control

Macrophages in a lymph node or tonsil.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa50-250) of human ANPEP protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

150kDa

Specificity & Comments

Recognizes an integral membrane glycoprotein of 150kDa, identified as CD13 (also known as aminopeptidase-N). The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal / tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Principe S, et al. J Proteome Res11:2386-96 (2012).

Bioinformatics

  •  Entrez Gene ID: 290
  •  Gene Symbol: ANPEP
  •  SwissProt: P15144
  •  Chromosome Location: 15q25-26
  •  Unigene: 1239