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Home >> Antibodies >> BCL11A / CTIP1 (Transcription Factor)

BCL11A / CTIP1 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-BCL11A-1G10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified BCL11A Mouse Monoclonal Antibody (PCRP-BCL11A-1G10). Confirmation of Integrity and Purity of Antibody.

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Flow cytometric analysis of PFA-fixed HeLa cells. BCL11A Mouse Monoclonal Antibody (PCRP-BCL11A-1G10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using BCL11A Mouse Monoclonal Antibody (PCRP-BCL11A-1G10) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PBX1-Monospecific Mouse Monoclonal Antibody (PCRP-BCL11A-1G10).

Product Details

Synonyms

B cell CLL/lymphoma 11A isoform 2; B-cell lymphoma/leukemia 11A; C2H2 type zinc finger protein; COUP-TF-interacting protein 1; CTIP1; Ecotropic viral integration site 9 protein homolog; EVI-9; Zinc finger protein 856; ZNF856

Positive Control

HeLa cells. Expressed in all tissues except in cells of the B and T lineage.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human BCLL1A protein

Cellular Localization

Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.

Species Reactivity

Human. Predicted to react in Mouse and Rat.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

91.2kDa

Specificity & Comments

Bcl-11a (CtIP-1, EVI9, B cell chronic lymphocytic leukemia (CLL)/lymphoma 11A) and Bcl-11b (CtIP-2, RIT1, B cell CLL/lymphoma 11B) genes play crucial roles in lymphopoiesis and influence the progression of hematopoietic malignancies. Disruption of the Bcl-11b locus is linked to T cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T cell lymphoma. Bcl-11 proteins are related C2H2 zinc-finger transcription factors that act as transcriptional repressors. Bcl-11b can interact with the metastasis-associated proteins MTA1 and MTA2 through the amino-terminal region. Bcl-11a is essential for postnatal development and normal lymphopoiesis. The Bcl-11a mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with Bcl-6.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 53335
  •  Gene Symbol: BCL11A
  •  SwissProt: Q9H165
  •  Chromosome Location: 2p16.1
  •  Unigene: 370549