2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> ALK (Anaplastic Lymphoma Kinase) / CD246

ALK (Anaplastic Lymphoma Kinase) / CD246

Recombinant Rabbit Monoclonal Antibody [Clone ALK1/2766R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Anaplastic LC Lymphoma stained with ALK-1 Recombinant Rabbit Monoclonal Antibody (ALK1/2766R).

img

SDS-PAGE Analysis Purified ALK Recombinant Rabbit Monoclonal Antibody (ALK1/2766R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

ALK Tyrosine Kinase Receptor, ALK/NPM1 fusion gene, Anaplastic lymphoma kinase Ki1, Anaplastic Lymphoma Kinase p80, anaplastic lymphoma receptor tyrosine kinase, CD246, mutant anaplastic lymphoma kinase, NBLST3

Positive Control

Anaplastic Large Cell Lymphoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ALK protein

Cellular Localization

Cytoplasmic and nuclear

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

80kDa (hybrid); 200kDa (wild type)

Specificity & Comments

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Pulford K, et al. Blood 1997;89:1394-404.

Bioinformatics

  •  Entrez Gene ID: 238
  •  Gene Symbol: ALK
  •  SwissProt: Q9UM73
  •  Chromosome Location: 2p23
  •  Unigene: 654469
NBT02766