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CD16 / FcγReceptor III

Mouse Monoclonal Antibody [Clone C16/1045]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified CD16 Mouse Monoclonal Antibody (C16/1045). Confirmation of Purity and Integrity of Antibody.

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF405S-labeled CD16 monoclonal antibody (C16/1045) (violet).

SDS-PAGE Analysis Purified CD16 Mouse Monoclonal Antibody (C16/1045). Confirmation of Purity and Integrity of Antibody.

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF405S-labeled CD16 monoclonal antibody (C16/1045) (violet).

Product Details

Synonyms

FcRIIIa; FCR-10; FcRIII; FCGRIII; FcR-10; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); neutrophil-specific antigen NA; CD16a; low affinity immunoglobulin gamma Fc region receptor III-A; Fc-gamma RIIIa; immunoglobulin G Fc receptor III; Fc-gamma RIII-alpha; Fc-gamma receptor IIIb (CD16); CD16a antigen; Fc-gamma receptor III-2 (CD16); FCGR3; Fc gamma receptor III-A; FCG3; low affinity III, receptor for (CD16); Fc-gamma RIII; IGFR3FcgammaRIIIA; CD16FCRIIIA; FCRIII; Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

Positive Control

K-562, hPBL or U-937 cells. Lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF More Details

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CD16 protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Others not-tested.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

50-80kDa

Specificity & Comments

It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.