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Home >> Antibodies >> IRF3

IRF3

Mouse Monoclonal Antibody [Clone PCRP-IRF3-6C8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed U87 cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Flow Cytometric Analysis of PFA-fixed Raji cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Flow Cytometric Analysis of PFA-fixed MCF-7 cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Flow Cytometric Analysis of PFA-fixed K562 cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Flow Cytometric Analysis of PFA-fixed HeLa cells. IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Western Blot Analysis of MCF-7 cell lysate using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8).

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Immunofluorescence Analysis of K562 cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain.

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Immunofluorescence Analysis of MCF-7 cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain.

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SDS-PAGE Analysis Purified IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8). Confirmation of Purity and Integrity of Antibody.

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Western blot analysis of HeLa cell lysate using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using IRF-3 Mouse Monoclonal Antibody (PCRP-IRF3-6C8).

Product Details

Synonyms

IIAE7; MGC94729; Interferon regulatory factor 3 (IRF3)

Positive Control

HeLa, U87 or Ramos cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human IRF3 protein

Cellular Localization

Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

50kDa

Specificity & Comments

Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-αand β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-αand interferon-βgenes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γp48 and IFN consensus sequence-binding protein (ICSBP).

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Chen, T., et al. 2018. Virus Genes 54: 514-526
  2. Lian, H., et al. 2018. Nat. Commun. 9: 3349.

Bioinformatics

  •  Entrez Gene ID: 3661
  •  Gene Symbol: IRF3
  •  SwissProt: Q14653
  •  Chromosome Location: 19q13.33
  •  Unigene: 289052