SDS-PAGE Analysis Purified Cytochrome C Mouse Monoclonal Antibody (6H2.B4). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Cytochrome C Mouse Monoclonal Antibody (6H2.B4) followed by Goat anti-mouse IgG-CF488 (Green).
Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytochrome C Mouse Monoclonal Antibody (6H2.B4) followed by Goat anti-mouse IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 is Control (Red).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.