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S100A1 (Melanoma Marker) Antibody [S1/61]

Additional information

Catalog Number: 6271-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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S100A1 (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S1/61)

S100A1 (Melanoma Marker) Antibody - Image 2

Flow Cytometric Analysis of T98G Cells. using S100A1 Mouse Monoclonal Antibody (S1/61) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

  • IF
In Stock
Catalog Number Formulation Availability Size Price
6271-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
6271-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
6271-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence
Species Reactivity
Human
Clone
S1/61
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
Gene Name
S100A1
Positive Control
T98G or A431 or HT-29 Cells. Brain or Melanoma
Immunogen
Purified human full-length native S-100 protein conjugated to methylated BSA
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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MICA Antibody

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MICA Antibody

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).
Key References
  • Vanstapel et al. 1986. Am J Clin Pathol. 85(2):160-8.
  • Vanstapel et al. 1985. Lab Invest. 52(2):232-8.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6271
SwissProt
P23297
Chromosome Location
1q21.3
Mol. Weight of Antigen
10-12kDa
Alternate Names
Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1, Bpb; NEF; S100-A1; S100 Alpha Chain; S100 Beta Chain; S100 Calcium Binding Protein A1; S100 Calcium Binding Protein B; S100 Calcium Binding Protein Beta Neural

Summary

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

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