Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Formalin-fixed, paraffin-embedded Rat Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Formalin-fixed, paraffin-embedded Mouse Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Western Blot Analysis of human kidney tissue lysate using KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Formalin-fixed, paraffin-embedded human Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Flow Cytometric Analysis of human HEK293 cells using KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

SDS-PAGE Analysis KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF640R (Purple)

Printable Datasheet

Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 [CDH16/1071]

Name: Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16
SKU: 1014-MSM1
Availability: InStock

Primary Antibodies Monoclonals Mouse Monoclonals

Summary

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Product Properties & Targets

Host

Mouse

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Species Reactivity

Dog, Human, Mouse, Rabbit, Rat

Research Areas

Apoptosis, Autophagy, Cancer

Isotype

IgG1

Cellular Localization

Cell membrane

Light Chain

Kappa

Gene Name

CDH16

Positive Control

HEK293 cells. Kidney tissue lysate. Normal kidney or renal cell carcinoma.

Immunogen

Recombinant full-length human CDH16 protein

Alternate Names

Cadherin-16, Kidney-specific cadherin, Cadherin-16 (CDH16); Kidney-specific cadherin; Ksp-cadherin antibody

Database Links

Entrez Gene ID

1014

Unigene

513660

SwissProt

O75309

Additional Information

Clone

CDH16/1071

Human Gene Symbol

CDH16

Chromosome Location

16q22.1

Mol. Weight of Antigen

130kDa

Functions

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Shen, S.S., et al. 2005. Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms. Mod Pathol. 18: 933-40
Mazal, P.R., et al. 2005. Expression of kidney-specific cadherin distinguishes chromophobe renal cell carcinoma from renal oncocytoma. Hum Pathol. 36: 22-28.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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